Hippokratia 2013, 17(2):141-145
Eleftheriadis T1, Pissas G1, Karioti A1, Antoniadi G1, Golfinopoulos S1, Liakopoulos V1, Mamara A2, M. Speletas M2, Koukoulis G3, Stefanidis I1
1Department of Nephrology, 2Department of Immunology, 3Department of Endocrinology, Medical School, University of Thessaly, Larissa, Greece
Background: Urate through Nacht Domain, Leucine-Rich Repeat, and pyrin domain-containing protein 3 (NALP3) dependent caspase-1 activation stimulates macrophages to secrete inteleukin-1β (IL-1β). Purinergic receptor P2X7 plays a role in the urate induced NALP3 activation. Urate also enhances adaptive immunity indirectly through its ef-fect on antigen presenting cells. In this study, the direct effect of urate on primary human lymphocytes was evaluated.
Methods: Lymphocytes were cultured with or without monosodium urate crystals in the presence or not of a P2X7 inhibitor. Caspase-1 activity was assessed colorimetrically in cell lysates and IL-1β was measured in supernatants with ELISA. Whole lymphocyte viability and proliferation, as well as T-cell proliferation were assessed by means of 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay and of flow cytometry respective-ly.
Results: Urate induced caspase-1 activation and IL-1β release by lymphocytes. It also induced proliferation of whole lymphocytes and T-cells as well. P2X7 inhibitor abrogated lymphocyte proliferation.
Conclusions: Urate, a well defined danger signal, stimulates directly human lymphocytes in a P2X7 dependent way. The subsequent IL-1β secretion could enhance inflammation, whereas expansion of lymphocyte clones could facilitate a subsequent adaptive immune response.
Key words: Uric acid, lymphocyte, caspase-1, interleukin-1β, P2X7
Corresponding author: Theodoros Eleftheriadis, MD, PhD, Department of Nephrology, Medical School, University of Thessaly, Neo Kti-rio, Mezourlo Hill, 41110 Larissa, Greece, tel: +302413501668, fax: +302413500242, e-mail: email@example.com
Uric acid is the end product of purine metabolism in humans and higher primates. Uric acid is currently under intense investigation by the immunologists since it acts as a danger associated molecular pattern (DAMP)1-3. Monosodium urate (MSU) crystals, the etiological agent of gout, are recognized by the Nacht Domain, Leucine-Rich Repeat, and pyrin domain-containing protein 3 (NALP3) in macrophages leading to inflammasome formation. Once inflammasome is formed, caspase-1 is activated and converts pro-interleukin-1β (pro-IL-1β) and pro-interleukin-18 (pro-IL-18) to active IL-1β and IL-18 respectively. Then these potent proinflammatory cytokines are secreted4.
The exact mechanism by which NALP3 is activated by MSU crystals in macrophages is not fully understood. Yet, the ability of NALP3 to sense various structurally diverse stimuli led to the hypothesis that it does not recognize each stimulus individually but senses a common downstream event5. According to a suggested mechanism, extracellular ATP binds to the purinergic receptor P2X7 leading to opening of this ATP-gated cation channel that induces potassium efflux and formation of a large pore by the hemichannel protein pannexin6,7. Intracellular potassium depletion is the trigger that activates NALP3, while inhibition of potassium efflux prevents NALP3 dependent caspase-1 activation in response to MSU crystals8,9.
An elegant study showed that released from dying cells uric acid induces inflammation. However, urate is not involved in the immune response to microbial stimuli or sterile irritant particles. These authors, focused on acetaminophen-induced hepatototoxicity, clearly showed that uric acid is an endogenous non redundant danger signal that regulates sterile inflammation in vivo10. Similarly in a bleomycin-induced lung injury model, released from injured cells uric acid constitutes a major DAMP that through NALP3 activation forms the inflammasome and induces IL-1β secretion11. The role of uric acid in inducing sterile inflammation is continuously extended. For example, a recent study showed that uric acid may play a role even in osteoarthritis, which traditionally is considered as a degenerative disease12.
Although uric acid is known to play a significant role in inflammation, less is known about its direct effect on the cells of adaptive immunity. Studies showed that uric acid promotes tumor immune rejection13, enhances T-cell immune response to antigens14 and plays important role in enhanced adaptive immunity by alum adjuvant15. However, these studies focused on the effect of uric acid on dendritic cells and not on its direct effect on lymphocytes. To our knowledge, there is only one study which showed that uric acid induces a subtle increase in the percentage of T-cells that express certain activation markers16. In the present study the direct effect of uric acid on primary human lymphocytes was evaluated.
Materials and Methods
Twelve healthy volunteers, 8 females and 4 males, from 20 to 47 years old, participated into the study. An informed consent was obtained from each individual and the hospital ethics committee gave its approval to the study protocol.
Lymphocyte isolation and culture
Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by Ficoll-Hypaque density gradient centrifugation (Histopaque 1077, Sigma-Aldrich, St. Louis, MO, USA). After washed with RPMI 1640 (Gibco BRL, Grand Island, NY, USA), mononuclear cells were seeded in 6 flat-bottom well plates and were incubated for 1h at 370C in a humidified atmosphere containing 5% CO2. Non-adherent lymphocytes were collected and counted by optical microscopy on a Neubauer plaque. Cell viability was assessed by trypan blue assay (Sigma-Aldrich) and the homogeneity of lymphocyte population was confirmed by means of optical microscopy.
Isolated lymphocytes were re-suspended in RPMI 1640 supplemented with 5% fetal bovine serum (Sigma-Aldrich) along with antibiotic-antimyotic solution (Sigma-Aldrich). Cells were cultured for 72h with or without uric acid (Sigma-Aldrich) at concentrations of 10 mg/dL or 20mg/dL and in the presence or not of 100nΜ KN-62 (Sigma-Aldrich), which at the given concentration inhibits selectively the purinergic receptor P2X717-19. Interestingly at both of the above concentrations urate was precipitated forming MSU crystals. All cultures were performed at 370C in a humidified atmosphere containing 5% CO2.
IL-1β secretion and Caspase -1 enzymatic activity assay
Lymphocytes (1 x 106/well) were cultured in flat-bottom 12 well plates for 72 h with or without uric acid at a concentration of 10 mg/dL or 20 mg/dL. IL-1β secreted in the supernatant was measured by means of ELISA (Quantikine Human IL-1β/IL-1F2, R&D Systems, Minneapolis, MN, USA). Caspase-1 enzymatic activity was assessed colorimetrically in lymphocyte protein extract. A kit based on the degradation of a caspase-specific peptide that is conjugated to the color reporter molecule p-nitroaniline (pNA) was used. The cleavage of the peptide by the caspase releases the chromophore pNA (Caspase -1 Colorimetric Assay, R&D Systems). All these experiments were performed in duplicates and the results refer to the mean of the two measurements.
Cell viability and proliferation assay
Lymphocytes (3 x 104/well) were cultured in flat-bottom 96 well plates and treated with uric acid in the presence or not of KN-62 for 72h. Viability and proliferation were assessed by measuring colorimetrically the cleavage of the XTT, a yellow tetrazolium salt, to the orange formazan dye via the succinate-tetrazolium reductase system in the mitochondria of metabolically active cells. For this purpose the TACS XTT cell proliferation assay kit (Trevigen, Gaithersburg, MD, USA) was used according to the manufacturer’s instructions. All these experiments were performed in triplicates and the results refer to the mean of the three measurements.
T-cell proliferation CFSE flow cytometry assay
Lymphocytes were stained with carboxyfluorescein succinimidyl ester (CFSE) (CellTrace, Invitrogen, Camarillo, CA, USA). CFSE passively diffuses into cells. It is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield highly fluorescent carboxyfluorescein succinimidyl ester. The succinimidyl ester group reacts with intracellular amines, forming fluorescent conjugates that are well retained, distributed equally between daughter cells, and the mean fluorescence halves accordingly. One µL of 5 mM stock solution was diluted in phosphate-buffered saline (PBS) (Sigma-Aldrich) and immediately added to the cultured lymphocytes (1×106 cells) in flat-bottom 12 well plates for 15 min at 37°C. Cells were then washed two times and cultured with or without uric acid at a concentration of 10 mg/dL.
After 72h in culture, lymphocytes were labeled with the phycoerythrin-Cy7 (PC7) conjugated mouse anti-CD3 monoclonal antibody (Beckman Coulter, Inc., Fullerton, CA, USA). Background was excluded with antihuman immunoglobulin IgG1 PC7 conjugated mouse isotypic control (Beckman Coulter). In brief, 100μl of stained with CFSE cultured lymphocytes were aliquoted into each tube. Anti-CD3 was added and incubated for 15 min at room temperature in darkness. Cells were washed with PBS (Sigma-Aldrich), pellet was resuspended with 500 μl PBS (Sigma-Aldrich) and analyzed within 1h in the Beckman Coulter FC500 apparatus.Although CD45 is highly useful for defining cultured blood cells, its utilization was not possible.
The normal distribution of the variables was evaluated and finally confirmed using the one-sample Kolmogorov-Smirnov test. Comparison of means was assessed by analysis of variance (ANOVA) followed by Bonferroni’s test. Results were expressed as mean ± SD and p<0.05 was considered statistically significant. Because in case of caspase-1 activity and XTT assay the colorimetric results were expressed as optical density (OD), comparison of OD means was performed, but the final results were expressed as % change from the control group.
Uric acid increases IL-1β secretion and caspase-1 activity in human primary lymphocytes
Uric acid at both concentrations of 10 mg/dL and 20 mg/dl increased markedly IL-1β secretion by human primary lymphocytes. IL1-β level at the supernatant was only 3.4 ± 1.03 pg/ml in untreated lymphocytes whereas its’ level was 148.83 ± 19.21 pg/ml in the presence of 10mg/dL uric acid and 156.46 ± 16.84 pg/ml in the presence of 20mg/dL uric acid (p<0.001 in both cases). Interestingly, there was not statistically significant difference in IL-1β levels secreted by lymphocytes treated with uric acid at a concentration of 10 mg/dL or at a concentration of 20 mg/dL (p=0.104) (Figure 1 left panel).
Figure 1: Uric acid induces IL-1β secretion (left panel) and caspase-1 activation (right panel) in primary human lymphocytes.
Uric acid at both concentrations of 10 mg/dL and 20 mg/dl increased significantly caspase-1 activity in lymphocyte cell lysates. Compared to untreated cells, caspase-1 activity was increased by 192 ± 19% in cells treated with uric acid at a concentration of 10 mg/dL (p=0.039) and by 241 ± 28% in cells treated with uric acid at a concentration of 20 mg/dL (p=0.05). Although caspase-1 activity was more increased in cells treated with uric acid at a concentration of 20 mg/dL, no statistically significant difference was reached compared to its activity in cells treated with uric acid at a concentration of 10 mg/dL (p=0.159) (Figure 1 right panel).
Uric acid induces purinergic receptor P2X7 dependent proliferation of primary human lymphocytes
Uric acid did not affect negatively cell viability. On the contrary, XTT assay revealed that uric acid induces lymphocyte proliferation. Compared to untreated lymphocytes, lymphocytes treated with uric acid at a concentration of 10 mg/dL showed an increased proliferation index of 171 ± 90% (p=0.016). Similarly, compared to untreated lymphocytes, lymphocytes treated with uric acid at a concentration of 20 mg/dL had an increased proliferation index of 214 ± 149% (p<0.001). Furthermore, compared to lymphocyte treated with uric acid at a concentration of 10 mg/dL, lymphocytes treated with uric acid at a concentration of 20 mg/dL had an increased proliferation index of 128 ± 49%, but this did not reach statistical significance (p=0.053) (Figure 2).
Figure 2: Uric acid induces proliferation of primary human lymphocytes, which is almost completely abrogated by the purinergic receptor P2X7 inhibitor KN62.
The purinergic receptor P2X7 inhibitor KN62 at the concentration of 100nM did not affect viability of lymphocytes, since the XTT assay derived optical density in KN62 treated lymphocytes was only 103 ± 14% compared to the derived optical density of untreated lymphocytes arbitrarily set as 100% (p=0.853). However, this compound affect uric acid induced lymphocyte proliferation significantly. More precisely, KN62 abrogates almost completely uric acid induced lymphocyte proliferation. Compared to untreated lymphocytes, lymphocytes treated with both uric acid at concentration of 10 mg/dL and KN62 had a proliferation index of 105±17% (p=0.561). Similarly, compared to untreated lymphocytes, lymphocytes treated with both uric acid at concentration of 20 mg/dL and KN62 had a proliferation index of 110 ± 26% (p=0.365) (Figure 2).
Uric acid induces T-cell proliferation
In order to define if uric acid induces T-cell proliferation we performed a CFSE based flow cytometry assay. In the presence of uric acid at a concentration of 10 mg/dL, CD3+ gated T-cells completed their expansion to the second generation, which was not achieved by the untreated CD3+ T-cells. This picture was reproducible in all performed experiments (Figure 3).
Figure 3: Flow cytometry CFSE assay in untreated or treated with urate CD3+ T-cells. Untreated T-cells failed to complete their expansion to the second generation (figure 3a). Treated with urate T-cells completed their expansion to the second generation (figure 3b). In flow cytometry dot-plots (left panels of figures 3a and 3b) the population of double positive cells with less CFSE intensity corresponds to the second generation of cells. Flow cytometry histograms of CFSE intensity of gated CD3+T-cells depict the complete expansion to the second generation of treated with urate T-cells (right panel of figure 3b), which was not achieved by untreated T-cells (right panel of figure 3a).
The effect of uric acid on macrophages and dendritic cells has been extensively studied4,13-15. The aim of this study was to evaluate the direct effect of uric acid on primary human lymphocytes, since the available data are scarce. For this reason we isolated and cultured lymphocytes from healthy volunteers.
Interestingly at the used concentrations urate was precipitated forming MSU crystals. It has been established previously, albeit in other cell types, that urate in the form of MSU crystals and not in its soluble form enhances inflammatory response20.
IL-1β secretion was measured in the supernatant and caspase-1 activity in cell lysates from lymphocytes treated or not with uric acid. Under uric acid treatment caspase-1 activity and IL-1β secretion were both markedly increased. Because NALP3 is expressed in both human B-cells21 and T-cells22 it is tempting to assume that like in macrophages4, urate acts in lymphocytes through the NALP3 dependent caspase-1 activation pathway. However, this remains to be elucidated further. By the way, the above result per se is intriguing, since it confirms that a DAMP can act directly in lymphocytes, inducing IL-1β secretion and possibly promoting inflammation independently of macrophages.
Another appealing result of the present study is that uric acid induces lymphocyte proliferation in the absence of an antigen receptor specific stimulus. This was confirmed with XTT assay for whole lymphocyte population, and with CFSE flow cytometry assay for T-cells specifically. The fact that the purinergic receptor P2X7 inhibitor KN62 abrogates almost completely uric acid induced lymphocyte proliferation indicates that not only in macrophages6-9 but possibly in lymphocytes too, NALP3 activation is mediated by the opening of this ATP-gated cation channel and the subsequent intracellular potassium depletion. The exact role of NALP3 in lymphocytes remains to be elucidated. The exact mechanism by which uric acid triggers the purinergic receptor P2X7 in lymphocytes remains to be elucidated as well. However, both T-cells and B-cells secrete ATP after various activating stimuli, such as T-cell antigen receptor binding or hypertonic stress in case of T-cells and Staphylococcus aureus Cowan I in case of B-cells. Then the released by viable cells ATP acts in an autocrine manner through the P2X receptors23-26.
A concern about monocyte contamination in lymphocyte cultures is rational, since a small monocyte contamination is inevitable after PBMC depletion of monocytes by adherence. However, the possible monocyte numbers are too small for giving any result in XTT assay (3×104 total cells/well) and caspase-1 assay (1×106 total cells/well) or for explain the IL-β concentrations (1×106 total cells/well). Certainly the results of the above assays correspond to lymphocyte proliferation, caspase-1 activation in lymphocytes and IL-1β production by lymphocytes. In addition to the very small number of monocytes, the fact that urate induces proliferation of T-cells in the absence of any T-cell antigen receptor stimulus excludes any role for this contamination.
An interesting question regarding the uric acid induced lymphocyte proliferation in the absence of antigen receptor triggering is raised from the results of the present study. It is well known that after antigen receptor triggering, activation of three transcription factors is essential for lymphocyte proliferation. These are the nuclear factor of activated T-cells (NFAT), activator protein-1 (AP-1) and the nuclear factor-κB (NF-κB)27,28. However, uric acid may be able to activate these transcription factors in lymphocytes. Uric acid induced IL-1β secretion acting in an autocrine way can bind IL-1β receptor and signal through myeloid differentiation factor 88 (MyD88) activating NF-κB and AP-129,30. Additionally, purinergic receptor P2X7 activation by uric acid could ultimately activate NFAT, since calcium influx through this ATP-gated channel is adequate for NFAT activation in lymphocytes31-33. Uric acid induced antigen receptor independent lymphocyte proliferation could then enhance a forthcoming adaptive immune response simply because of the expansion of lymphocytes clones. Thus, the direct effect of uric acid on lymphocytes may contribute to the attributed to it enhanced adaptive immune response.
In conclusion, urate crystals, a well defined DAMP, stimulate human lymphocytes in a purinergic receptor P2X7 dependent way. The subsequent IL-1β secretion could enhance inflammation, whereas expansion of lymphocyte clones could facilitate a subsequent adaptive immune response. These results support the significant role of urate in shaping adaptive immune response not only indirectly through antigen presenting cells, but also directly through its action on lymphocytes. In the clinical field urate application as an adjuvant in vaccines and the effect of pharmaceutical alteration of its levels on immune related disorders are subjects for further evaluation.
Declaration of interest
1. Shi Y, Evans JE, Rock KL. Molecular identification of a danger signal that alerts the immune system to dying cells. Nature. 2003; 425: 516-521.
2. Bianchi ME. DAMPs, PAMPs and alarmins: all we need to know about danger. J Leukoc Biol. 2007; 81: 1-5.
3. Ferguson TA, Choi J, Green DR. Armed response: how dying cells influence T-cell functions. Immunol Rev. 2011; 241: 77-88.
4. Martinon F, Pétrilli V, Mayor A, Tardivel A, Tschopp J. Gout-associated uric acid crystals activate the NALP3 inflammasome. Nature. 2006; 440: 237-241.
5. Chen GY, Nuñez G. Sterile inflammation: sensing and reacting to damage. Nat Rev Immunol. 2010; 10: 826-837.
6. Pelegrin P, Surprenant A. Pannexin-1 mediates large pore formation and interleukin-1beta release by the ATP-gated P2X7 receptor. EMBO J. 2006; 25: 5071-5082.
7. Locovei S, Wang J, Dahl G. Activation of pannexin 1 channels by ATP through P2Y receptors and by cytoplasmic calcium. FEBS Lett. 2006; 580: 239-244.
8. Petrilli V, Papin S, Dostert C, Mayor A, Martinon F, Tschopp J. Activation of the NALP3 inflammasome is triggered by low intracellular potassium concentration. Cell Death Differ. 2007; 14: 1583-1589.
9. Cassel SL, Eisenbarth SC, Iyer SS, Sadler JJ, Colegio OR, Tephly LA, et al. The Nalp3 inflammasome is essential for the development of silicosis. Proc Natl Acad Sci U S A. 2008; 105: 9035-9040.
10. Kono H, Chen CJ, Ontiveros F, Rock KL. Uric acid promotes an acute inflammatory response to sterile cell death in mice. J Clin Invest. 2010; 120: 1939-1949.
11. Gasse P, Riteau N, Charron S, Girre S, Fick L, Pétrilli V, et al. Uric acid is a danger signal activating NALP3 inflammasome in lung injury inflammation and fibrosis. Am J Respir Crit Care Med. 2009; 179: 903-913.
12. Denoble AE, Huffman KM, Stabler TV, Kelly SJ, Hershfield MS, McDaniel GE, et al. Uric acid is a danger signal of increasing risk for osteoarthritis through inflammasome activation. Proc Natl Acad Sci U S A. 2011; 108: 2088-2093.
13. Hu DE, Moore AM, Thomsen LL, Brindle KM. Uric acid promotes tumor immune rejection. Cancer Res. 2004; 64: 5059-5062.
14. Ma XJ, Tian DY, Xu D, Yang DF, Zhu HF, Liang ZH, et al. Uric acid enhances T cell immune responses to hepatitis B surface antigen-pulsed-dendritic cells in mice. World J Gastroenterol. 2007; 13: 1060-1066.
15. Kool M, Soullié T, van Nimwegen M, Willart MA, Muskens F, Jung S, et al. Alum adjuvant boosts adaptive immunity by inducing uric acid and activating inflammatory dendritic cells. J Exp Med. 2008; 205: 869-882.
16. Webb R, Jeffries M, Sawalha AH. Uric acid directly promotes human T-cell activation. Am J Med Sci. 2009; 337: 23-27.
17. Wiley JS, Gargett CE, Zhang W, Snook MB, Jamieson GP. Partial agonists and antagonists reveal a second permeability state of human lymphocyte P2Z/P2X7 channel. Am J Physiol. 1998; 275: C1224-C1231.
18. Humphreys BD, Virginio C, Surprenant A, Rice J, Dubyak GR. Isoquinolines as antagonists of the P2X7 nucleotide receptor: high selec-tivity for the human versus rat receptor homologues. Mol Pharmacol. 1998; 54: 22-32.
19. Gargett CE, Wiley JS. The isoquinoline derivative KN-62 a potent antagonist of the P2Z-receptor of human lymphocytes. Br J Pharma-col. 1997; 120: 1483-1490.
20. Kobayashi T, Kouzaki H, Kita H. Human eosinophils recognize endogenous danger signal crystalline uric acid and produce proinflamma-tory cytokines mediated by autocrine ATP. J Immunol. 2010; 184: 6350-6358.
21. Kolly L, Busso N, Palmer G, Talabot-Ayer D, Chobaz V, So A. Expression and function of the NALP3 inflammasome in rheumatoid synovium. Immunology. 2010; 129: 178-185.
22. Petterson T, Månsson A, Riesbeck K, Cardell LO. Nucleotide-binding and oligomerization domain-like receptors and retinoic acid induc-ible gene-like receptors in human tonsillar T lymphocytes. Immunology. 2011; 133: 84-93.
23. Schenk U, Westendorf AM, Radaelli E, Casati A, Ferro M, Fumagalli M, et al. Purinergic control of T cell activation by ATP released through pannexin-1 hemichannels. Sci Signal. 2008; 1: ra6.
24. Schenk U, Frascoli M, Proietti M, Geffers R, Traggiai E, Buer J, et al. ATP inhibits the generation and function of regulatory T cells through the activation of purinergic P2X receptors. Sci Signal. 2011; 4: ra12.
25. Woehrle T, Yip L, Manohar M, Sumi Y, Yao Y, Chen Y, et al. Hypertonic stress regulates T cell function via pannexin-1 hemichannels and P2X receptors. J Leukoc Biol. 2010; 88: 1181-1189.
26. Sakowicz-Burkiewicz M, Kocbuch K, Grden M, Maciejewska I, Szutowicz A, Pawelczyk T. High glucose concentration impairs ATP outflow and immunoglobulin production by human peripheral B lymphocytes: involvement of P2X7 receptor. Immunobiology. 2013; 218: 591-601.
27. Huang Y, Wange RL. T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004; 279: 28827-28830.
28. Maddaly R, Pai G, Balaji S, Sivaramakrishnan P, Srinivasan L, Sunder SS, et al. Receptors and signaling mechanisms for B-lymphocyte activation, proliferation and differentiation–insights from both in vivo and in vitro approaches. FEBS Lett. 2010; 584: 4883-4894.
29. Sims JE, Smith DE. The IL-1 family: regulators of immunity. Nat Rev Immunol. 2010; 10: 89-102.
30. Barksby HE, Lea SR, Preshaw PM, Taylor JJ. The expanding family of interleukin-1 cytokines and their role in destructive inflammatory disorders. Clin Exp Immunol. 2007; 149: 217-225.
31. Yip L, Woehrle T, Corriden R, Hirsh M, Chen Y, Inoue Y, et al. Autocrine regulation of T-cell activation by ATP release and P2X7 receptors. FASEB J. 2009; 23: 1685-93.
32. Woehrle T, Yip L, Elkhal A, Sumi Y, Chen Y, Yao Y, et al. Pannexin-1 hemichannel-mediated ATP release together with P2X1 and P2X4 receptors regulate T-cell activation at the immune synapse. Blood. 2010; 116: 3475-3484.
33. Junger WG. Immune cell regulation by autocrine purinergic signalling. Nat Rev Immunol. 2011; 11: 201-212.