Hippokratia 2017, 21(3):124-129
Tsiroukidou K1, Papagianni M1, Hatziagorou E1, Galli-Tsinopoulou A2, Giannopoulos A3, Tsanakas I1
13rd Department of Pediatrics, Hippokration General Hospital, 24th Department of Pediatrics, Papageorgiou General Hospital, 32nd Department of Pediatrics, AHEPA University General Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece
Background: Childhood obesity poses a global health threat. We investigated the association of the cardiopulmonary exercise testing indexes with adipokines levels and insulin resistance along with the beneficial effect of physical exercise on insulin resistance in children.
Material and Methods: Thirty-two obese, 21 overweight, and 30 normal-weight children participated in the current study, with mean age 11.98 (±1.95), 10.91 (±1.72), and 11.35 (±2.21) years, respectively. All children were clinically healthy. The children and their parents provided data on physical activity, while spirometry and maximal cardiopulmonary exercise testing were performed for the functional evaluation of the respiratory status of the study population.
Results: Leptin levels were significantly lower in normal-weight children compared to the obese ones (p <0.001). Maximum quantity of oxygen (VO2max) differences were statistically significant between the three groups (p =0.025 for normal weight vs overweight, and p =0.001 for normal vs obese children). Leptin levels were inversely related to VO2max in obese children (p =0.009, r =-0.491). Homeostatic model assessment of insulin resistance (HOMA-IR) was statistically significantly lower among children that were more physically active (p =0.042). Leptin was significantly related to body mass index among obese children (r =-0.582, p <0.001).
Conclusions: Leptin is significantly inversely related to VO2max in obese children. This study, however, allows further assumptions for adipokines and childhood obesity, along with the possible role of leptin as an additional obesity index in relation with cardiopulmonary function. HIPPOKRATIA 2017, 21(3): 124-129.
Key words: Obesity, childhood, cardiopulmonary exercise testing, leptin, adipokines
Corresponding author: Tsiroukidou Kyriaki, 3rd Department of Pediatrics, Hippokration General Hospital, 49 Konstantinoupoleos Str, 54642, Thessaloniki, tel: +306945285739, email: firstname.lastname@example.org
Obesity which is defined as abnormal or excessive fat accumulation in the adipose tissue threatens the health of the individuals1. Especially childhood obesity poses an evolving global health threat1. The obesity rate for children aged 6-11 and 12-19 years has increased since 1980 from 7 % to 20 % and from 5 % to 18 %, respectively2,3. However, in some western settings such as the US, Western Europe, Australia, and Japan, recent data suggest that levels of childhood obesity may have reached a plateau in the last decade4. This fact should not lead to complacency, as one-third of adults and almost 20 % of children and adolescents 2-19 years old are obese.
Excess body weight poses a health risk for premature morbidity and mortality. Diseases such as type 2 diabetes, dyslipidemia, hypertension, cardiovascular disease, and sleep apnea have recently become more common in children5. Circulating adipokines are correlated with obesity, and their levels have been related to insulin resistance6,7. Among them, leptin and adiponectin are the significant adipocytokines through which obesity exerts its clinical and metabolic effects7. Leptin which is mainly produced in the adipose tissue, acts on the hypothalamus, on receptors in the arcuate nucleus, to regulate appetite in order to be achieved energy homeostasis8. A decreased sensitivity to leptin has been noticed in obesity, resulting in an inability to detect satiety despite high energy stores9. Serum leptin levels were found elevated in obese children; besides, leptin levels decreased during the weight loss period10.
Aerobic Capacity with all its indices as derived from the maximal exercise testing, has been used as a prognostic factor in many conditions11-13. Especially the maximum quantity of oxygen (VO2max) that a person’s lungs consume during maximal exercise, has been recognized as the gold standard prognostic marker in several diseases. Shazia et al showed that obese young women presented lower aerobic capacity than normal-weight ones with lower VO2max values in the obese group14. Moreover, in a systematic review and meta-analysis on exercise tolerance in adolescents, Hansen et al marked a trend for lower VO2peak in obese adolescents than lean ones and highlighted the need for more studies in this field15.
The child’s cardiopulmonary capacity, as documented by the cardiopulmonary exercise testing (CPET) indexes, reflects the pulmonary, cardiovascular, muscle, and metabolic responses simultaneously under precise conditions of metabolic stress. The latter is also expressed via changes in adipokines levels, which might be associated with CPET indexes16.
The aim of the present study was to investigate the associations of the cardiopulmonary exercise testing indexes with adipokines levels and insulin resistance and the impact of physical exercise on insulin resistance in children.
Material and Methods
We conducted a prospective study from 2009 until 2013 which enrolled 53 obese and overweight children who attended the Obesity clinic of the Endocrine Unit of the 3rd Department of Pediatrics of the Aristotle University of Thessaloniki at Hippokration General Hospital of Thessaloniki (overweight and obese groups) and 30 children with similar age and sex with the above two groups, who attended two different public schools in the city of Thessaloniki (normal-weight group). Normal-weight children were enrolled and participated in the study after they and their parents were informed about the study protocol, through an information leaflet that was sent to their schools. In total, 33 normal weight children accepted to participate in the study from the 123 children that were informed about it. The normal-weight group did not differ from the other groups regarding age and sex. Three normal weight children and two obese were excluded from the study. The exclusion criteria for the study were: a) chronic diseases, and b) failure to perform a cardiopulmonary exercise test.
The study was approved by the Bioethics Committee of the Medical School of Aristotle University of Thessaloniki (decision number: 91/21-12-2009), and a signed consent form by the guardian was obtained for each single child.
In the current study, 32 obese, 21 overweight, and 30 normal-weight children participated, with a mean age (± standard deviation) 11.98 ± 1.95, 10.91 ± 1.72, and 11.35 ± 2.21 years, respectively. All children were clinically healthy. The children and their parents provided data on physical activity. They were asked about in-school physical activity, as defined in their curriculum and out-of-school activity. The latter was considered to be “organized” only if the child participated in certain sports or attended a gym. Even though all “physical activity” hours were recorded, only children with “organized” physical activity, were considered as “physically active”. All children reported a 2-hour per week in-school activity.
Spirometry and maximal cardiopulmonary exercise testing were performed at the Pulmonology Unit of the 3rd Department of Pediatrics.
We used the SECA scale, model 812 (S.No 06901304) to measure the children’s weight and the Holten Limited measuring rod (V/R, Veeder-Root, Crymych, Dyfed, UK) to measure their height. The growth curves for the Greek population designed by the First Paediatric Department of the University of Athens in 2000-2001, were used to determine the percentile of weight and height.
Spirometry was performed with an electronic volume spirometer, dry type (SensorMedics, Flow Sensor, Vmax Series, V.20-1 L, SensorMedics Corp., Yorba Linda, CA, USA). We measured the forced vital capacity (FVC), forced expiratory volume in one second (FEV1), and forced expiratory flow at 50 % of FVC (FEF50), using standard spirometry following the ATS/ERS guidelines17. No beta-2 agonists were used for 24 hours before spirometry. Data were expressed in % predicted using the normative data from the Global Lung Function Initiative software (GLI 2012, Global Lung Function Initiative Task Force, available at: http://www.lungfunction.org/).
Glucose was measured by the hexokinase method (AU 2700 Analyzer, Beckman Coulter Inc., Orange County, CA, USA) and insulin by the two sites immunoassay technique (Immulite 2000 XPi Immunoassay System, Siemens Healthcare GmbH, Erlangen, Germany). Homeostatic model assessment of insulin resistance (HOMA-IR) was calculated with the following formula: HOMA-IR = Glucose (mg/dL) x insulin (mU/L)/405.
Regarding adipokines, leptin and adiponectin were measured with the “two-site” sandwich enzyme-linked immunosorbent assay (ELISA). Homocysteine was measured with electrochemical fluorescence (Centaur CP, Siemens Healthcare GmbH).
Cardiopulmonary Exercise Testing (CPET)
All participants performed a maximal cardiopulmonary exercise testing on a cycle ergometer (Ergoline, Vmax Series V.20-1, SensorMedics). Cardiac parameters were also measured (cardiograph model Corina, S.N. 101164361, Cardiosoft software V5.15). The Godfrey protocol for exercise testing was applied18; depending on subject’s height, after baseline measurements for one minute and a warm-up period of two minutes cycling with 10 Watts (for patients <120 cm tall), 15 Watts (120-150 cm) or 20 Watts (>150 cm tall), workload was increased by 10, 15, 20 Watts, respectively every minute until volitional fatigue. Exercise testing time was kept within 8-12 min. Patient’s heart rate over 85 % of maximum predicted19 along with respiratory equivalence ratio (RER) over 1.0520 were used as indicators of maximal test. Maximum oxygen uptake (VO2max), and max oxygen uptake/weight (VO2max/kg) were measured.
Descriptive and inferential statistics were performed. The out-of-school organized physical activity was considered as a binary variable around its median value (two hours weekly). Student’s t-test and ANOVA with post-hoc Bonferroni t-test were used for the two groups and multiple comparisons, respectively. Pearson’s r was used for correlations. Statistical significance was set at p =0.05. Statistical analyses were performed using the IBM SPSS Statistics for Windows (IBM SPSS, IBM Corp., Armonk, NY, USA) version 22.0 while for graphs we used the Sigma Plot 12.5 (Systat Software, London, UK).
The demographic and laboratory data of the three study groups are presented in Table 1. Statistically significant differences were detected in all studied parameters, except homocysteine, as shown in Table 1. Post-hoc analysis showed that leptin levels were significantly lower in normal-weight compared to obese children (p <0.001) while the difference between overweight and obese children was marginally statistically significant (p =0.05). The VO2max differences were also statistically significant between the three groups (p =0.025 for normal-weight vs overweight, and p =0.001 for normal-weight vs obese children). The levels of leptin and adiponectin among the three study groups are shown in Figure 1, while the VO2max levels are showed in Figure 2. Body mass index (BMI) z-scores also differed significantly between the three groups. When adipokines and VO2max (absolute values and adjusted for weight and height) were correlated with one another, significant correlations merged in overweight and obese children groups. Leptin was inversely related to VO2max in obese children (r =-0.491, p =0.009) as shown in Table 2. Leptin was also related inversely to VO2max/w (r =-0.441, p =0.021), to VO2max/h (r =-0.420, p =0.029), and to VO2max (r =-0.491, p =0.009) only in obese children. However, when these correlations were corrected for HOMA-IR and BMI z-scores, only BMI z-score was positively related to leptin (p =0.014 for VO2max/w and p =0.011 for VO2max/h regression model respectively). As for homocysteine, no statistically significant associations were detected (Table 2). Physical activity (organized or not) was not related statistically significantly to VO2max (p >0.10 for all indices) as shown in Table 3.
Figure 1: Leptin and adiponectin distribution in the obese, overweight, and normal-weight children. Statistical differences are evident between normal and obese group*.
*Leptin differed statistically significantly between normal-weight and overweight (p =0.05), normal-weight and obese group (p <0.001).
Figure 2: VO2max differences between groups (obese, overweight, and normal-weight children)*.
*VO2max differed statistically significantly between normal weight and overweight group (p =0.025) and between normal weight and obese group (p =0.001).
Leptin was significantly higher in the more physically active group (p =0.002) (Table 3). No associations were detected between leptin, adiponectin, and HOMA-IR or between these two adipokines and the BMI, with the exception of the obese children where leptin was significantly related to BMI (r =0.582, p <0.001) and HOMA-IR positively related to FEV1/FVC.
The main finding of the present study was that leptin was significantly inversely related to VO2max, adjusted for weight or height. This relation was subtle in overweight children and became evident in obese ones. Moreover, a documented, “organized” physical exercise duration of more than two hours weekly was associated with a lower HOMA-IR. Physical training may further exert its beneficial effect on health as documented by HOMA-IR index.
The VO2max associations with adipokines’ levels have been previously investigated and yielded contradictory results, mainly due to different research protocols. In the study of Unal et al, VO2max values were significantly higher (p <0.01) and leptin levels significantly lower in trained athletes21, while Yang et al showed that leptin levels after 24h of participating in an exercise bout of 65 % VO2max compared to 25 % were significantly higher22. Torjman et al did not find a change in serum leptin levels neither after short-term maximal exercise nor after four hours following long-term aerobic exercise at 50 % of VO2max23. Leptin levels have been positively correlated with BMI in both genders, but negatively correlated with VO2max only in men24. In the study of Paltoglou et al, baseline and post-exercise (at 70 % VO2 max) adiponectin was greater, and leptin and high-sensitivity C-reactive protein were lower in normal-weight than in obese pre- and early pubertal boys, authors concluding that leptin and adiponectin predict negatively and positively anti-oxidation, respectively25. Cardiopulmonary capacity studies in children are scarce, mainly because cardiopulmonary testing in children is particularly difficult and demanding, as the child’s cooperation is necessary to get maximum capacity.
In our study, leptin was significantly related to BMI only in obese children, while no associations were documented between these two adipokine levels and the insulin resistance. However, while several adipokines have been associated with obese and insulin-resistant phenotypes, a select group has been linked with insulin sensitivity, namely leptin, adiponectin. In the study of Plonka et al, the degree of body fat and the body composition (as expressed by BMI and other metabolic markers) were proportional to leptin concentration26. The increase of leptin levels in obese children normalized after weight loss, while leptin levels were associated with insulin resistance. In another study, all adipocytokines but TNF-a showed significant correlations (negative for adiponectin and positive for others) with clinical biomarkers of obesity and its complications: z-score for BMI, systolic and diastolic blood pressure. Adiponectin and leptin levels were also negatively and positively respectively related to central adiposity quantified by waist circumference. Leptin is the only adipokine associated with insulin resistance in children, while leptin, resistin, and IL-6 have been found to vary significantly different in obese children compared to normal weight controls27. The low physical activity in subjects with abdominal obesity has been independently and inversely linked to plasma leptin and insulin sensitivity.
There is also growing evidence that exercise exerts its beneficial effects partly through alterations in the adipokine profile; that is, exercise increases secretion of anti-inflammatory adipokines and reduces proinflammatory cytokines. In the present study, leptin levels were related to physical activity duration only in normal weight group, and HOMA-IR differed between the normal weight and obese children. Okazaki et al concluded that short-term and intensive exercise results in lower leptin concentration, regardless of changes in adipose tissue mass28. It has been found that there is a significant difference in physical activity levels between normal weight and obese/overweight adolescents.
Our results indicate that adipokines’ levels may reflect cardiopulmonary capacity and they could be markers of insulin resistance. However, the small number of participants in the present study, along with the fact that information on physical exercise in the present was based on parental and children reports, do not allow generalization of our findings. The number of the children who volunteered to cardiopulmonary testing was the main sample limitation. Moreover, outcomes might be different if there was a daily recording of exercise by independent observers. A limitation of the current study is the fact that the obesity status of the participating children was determined simply by using their BMI instead of performing a dual-energy X-ray absorptiometry (DEXA) scan. Another limitation is that currently there is a newer version of HOMA-IR available (can be accessed at the University of Oxford website).
In conclusion, this is the first study to the best of our knowledge that associates the VO2max with leptin and adiponectin in children. Innate difficulties in cardiopulmonary testing in children may account for data paucity in this age group. An interdisciplinary approach and excellent cooperation of the children is necessary. Our study allows further assumptions for adipokines and childhood obesity, along with providing information on the use of leptin as a possible additional obesity index in the future.
We thank our colleagues Kalliope Kontouli and Asterios Kampouras for assistance with the particular technique about cardiopulmonary exercise testing and Konstantina Kosta who provided insight and expertise that greatly assisted this study.
Conflict of interest
The authors have no conflict of interest to report.
1. Lobstein T, Baur L, Uauy R; IASO International Obesity TaskForce. Obesity in children and young people: a crisis in public health. Obes Rev. 2004 ;5 Suppl 1: 4-104.
2. Ogden CL, Carroll MD, Kit BK, Flegal KM. Prevalence of childhood and adult obesity in the United States, 2011-2012. JAMA. 2014;311:806-814.
3. Lakshman R, Elks CE, Ong KK. Childhood obesity. Circulation. 2012; 126: 1770-1779. 4. de Onis M, Blössner M, Borghi E. Global prevalence and trends of overweight and obesity among preschool children. Am J Clin Nutr. 2010; 92: 1257-1264.
5. Lee YS. Consequences of childhood obesity. Ann Acad Med Singapore. 2009; 38: 75-77.
6. Martos-Moreno GÁ, Barrios V, Chowen JA, Argente J. Adipokines in childhood obesity. Vitam Horm. 2013; 91: 107-142.
7. Al Mutairi S, Mojiminiyi OA, Al Alawi A, Al Rammah T, Abdella N. Study of leptin and adiponectin as disease markers in subjects with obstructive sleep apnea. Dis Markers. 2014; 2014: 706314.
8. Brennan AM, Mantzoros CS. Drug Insight: the role of leptin in human physiology and pathophysiology-emerging clinical ap-plications. Nat Clin Pract Endocrinol Metab. 2006; 2: 318-327.
9. Pan H, Guo J, Su Z. Advances in understanding the interrelations between leptin resistance and obesity. Physiol Behav. 2014; 130: 157-169.
10. Dubey S, Kabra M, Bajpai A, Pandey RM, Hasan M, Gautam RK, et al. Serum leptin levels in obese Indian children relation to clinical and biochemical parameters. Indian Pediatr. 2007; 44: 257-262.
11. Myers J, Arena R, Cahalin LP, Labate V, Guazzi M. Cardiopulmonary Exercise Testing in Heart Failure. Curr Probl Cardiol. 2015; 40: 322-372.
12. Parazzi PL, Marson FA, Ribeiro MA, Schivinski CI, Ribeiro JD. Ventilatory Efficiency in Children and Adolescents: A Sys-tematic Review. Dis Markers. 2015; 2015: 546891.
13. Nguyen S, Leroy S, Cracowski C, Perez T, Valette M, Neviere R, et al. [Prognostic value of clinical exercise testing in adult patients with cystic fibrosis]. Rev Mal Respir. 2010; 27: 219-225.
14. Shazia SM, Badaam KM, Deore DN. Assessment of aerobic capacity in overweight young females: A cross-sectional study Int J Appl Basic Med Res. 2015; 5: 18-20.
15. Hansen D, Marinus N, Remans M, Courtois I, Cools F, Calsius J, et al. Exercise tolerance in obese vs. lean adolescents: a systematic review and meta-analysis. Obes Rev. 2014; 15: 894-904.
16. Raj M. Obesity and cardiovascular risk in children and adolescents. Indian J Endocrinol Metab. 2012; 16: 13-19.
17. Miller MR, Hankinson J, Brusasco V, Burgos F, Casaburi R, Coates A, et al; ATS/ERS Task Force. Standardisation of spi-rometry. Eur Respir J. 2005; 26: 319-338.
18. Godfrey S, Davies CT, Wozniak E, Barnes CA. Cardio-respiratory response to exercise in normal children. Clin Sci. 1971; 40: 419-431.
19. American Thoracic Society; American College of Chest Physicians. ATS/ACCP Statement on cardiopulmonary exercise testing Am J Respir Crit Care Med. 2003; 167: 211-277.
20. Orenstein D. Assessment of exercise pulmonary function. Rowland TW (ed). Pediatric Laboratory Exercise Testing Clinical Guidelines. Champaign, IL: Human Kinetics, 1993, 141-163.
21. Unal M, Unal DO, Baltaci AK, Mogulkoc R. Investigation of serum leptin levels and VO2max value in trained young male athletes and healthy males. Acta Physiol Hung. 2005; 92: 173-179.
22. Yang CB, Chuang CC, Kuo CS, Hsu CH, Tsao TH. Effects of an acute bout of exercise on serum soluble leptin receptor (sOB-R) levels. J Sports Sci. 2014; 32: 446-451.
23. Torjman MC, Zafeiridis A, Paolone AM, Wilkerson C, Considine RV. Serum leptin during recovery following maximal in-cremental and prolonged exercise. Int J Sports Med. 1999; 20: 444–450.
24. Maïmoun L, Simar D, Caillaud C, Coste O, Puech AM, Jaussent A, et al. Basal plasma leptin levels in healthy elderly males are related to physical fitness without impact on bone metabolism. J Sports Med Phys Fitness. 2011; 51: 160-168.
25. Paltoglou G, Schoina M, Valsamakis G, Salakos N, Avloniti A, Chatzinikolaou A, et al. Interrelations among the adipocyto-kines leptin and adiponectin, oxidative stress and aseptic inflammation markers in pre- and early-pubertal normal-weight and obese boys. Endocrine. 2017; 55: 925-933.
26. Plonka M, Toton-Morys A, Adamski P, Suder A, Bielanski W, Dobrzanska MJ, et al. Association of the physical activity with leptin blood serum level, body mass indices and obesity in schoolgirls. J Physiol Pharmacol. 2011; 62: 647-656.
27. Gherlan I, Vladoiu S, Alexiu F, Giurcaneanu M, Oros S, Brehar A, et al. Adipocytokine profile and insulin resistance in childhood obesity. Maedica (Buchar). 2012; 7: 205-213.
28. Okazaki T, Himeno E, Nanri H, Ogata H, Ikeda M. Effects of mild aerobic exercise and a mild hypocaloric diet on plasma leptin in sedentary women. Clin Exp Pharmacol Physiol. 1999; 26: 415-420.