Hippokratia 2001, 5(1):13-22
AP Kambaroudis, Th Gerassimidis, D Karamanos, Ch Passinis, Ath Sakadamis
The purpose of this study was: a) the evaluation of the changes of the values of GOT (glutamine-transaminase), GPT (glutamine-pyruvate transaminase) and CPK (creatine – phosphokinase), during a 12-hour – period of acute muscle tissue ischemia and corresponding time of reperfusion, and b) the investigation of utilizing these changes and the evaluation of the degree of ischemic lesions and the restoration of them after reperfusion.
Methods – Material: In the right posterior limb of 15 non thorough – canines we provoked acute ischemia lasting 12 hours that was followed by reperfusion of the corresponding time by removing the cause of obstruction. Before the introduction of ischemia, 6 hours after it, 12 hours after it, 6 hours after reperfusion and 12 hours after reperfusion we obtained blood samples from systemic circulation (healthy limb was used as reference values) and from the venous circulation of the suffering limb and samples from muscle tissue from the healthy limb that was used as reference value and from the suffering limb for the evaluation of GOT, GPT and CPK.
Results: GOT and GPT in systemic circulation and in the intracellular space of the healthy muscle tissue did not show consideIable changes. In venous blood and in the intracellular space of the suffering limb during the period of acute ischemia we observed a statistically non-important increase of GOT and GPT which was observed during reperfusion as well. CPK in systemic circulation in venous blood of the suffering limb and in suffering muscle tissue was increased considerably during ischemia and remained increased during reperfusion.
Conclusion: The changes of GOT and GPT in systemic circulation and in the intracellular space of healthy muscle tissue and in the suffering limb did not correlate to the degree of ischemic changes ?or to the changes after reperfusion. The changes of CPK in systemic circulation and in venous blood of the suffering limb can be used selectively for the evaluation of the ischemic lesion. The change in CPK in the intracellular space of the muscle tissue of the suffering limb cannot be used for the evaluation of the degree of the ischemic lesion.